一、概述: |
一般來說,雄性(xing)鴿(ge)(ge)子(zi)(zi)具有(you)ZZ染色體(ti),而雌性(xing)鴿(ge)(ge)子(zi)(zi)則具有(you)ZW染色體(ti)。鴿(ge)(ge)子(zi)(zi)的(de)性(xing)別(bie)(bie)通(tong)常無法(fa)憑肉(rou)眼外(wai)觀直接(jie)確定(ding),因(yin)為(wei)它們沒有(you)明顯的(de)性(xing)別(bie)(bie)特(te)征。然而,通(tong)過基(ji)因(yin)檢測(ce)提取鴿(ge)(ge)子(zi)(zi)的(de)DNA樣本,可(ke)以(yi)進行基(ji)因(yin)檢測(ce)來確定(ding)其性(xing)別(bie)(bie)。這種方法(fa)利用了鴿(ge)(ge)子(zi)(zi)的(de)性(xing)染色體(ti)差異。通(tong)過檢測(ce)特(te)定(ding)基(ji)因(yin)片(pian)段的(de)存在與否(fou),可(ke)以(yi)確定(ding)鴿(ge)(ge)子(zi)(zi)的(de)性(xing)別(bie)(bie)。
二、儀器基本結構: |
鴿(ge)子(zi)性(xing)別檢(jian)(jian)測儀(yi)是實(shi)時(shi)檢(jian)(jian)測反應(ying)的(de)儀(yi)器(qi),主(zhu)要(yao)(yao)由(you)基(ji)(ji)因(yin)(yin)擴(kuo)增熱(re)(re)循(xun)環(huan)系(xi)(xi)統(tong)(tong)(tong)(tong)、熒(ying)(ying)光實(shi)時(shi)檢(jian)(jian)測系(xi)(xi)統(tong)(tong)(tong)(tong)、微電路(lu)控制(zhi)系(xi)(xi)統(tong)(tong)(tong)(tong)、計算機及應(ying)用軟(ruan)件(jian)組(zu)成(cheng)。其中(zhong)兩個(ge)核心(xin)功能模塊(kuai):熱(re)(re)循(xun)環(huan)系(xi)(xi)統(tong)(tong)(tong)(tong)和(he)熒(ying)(ying)光實(shi)時(shi)檢(jian)(jian)測系(xi)(xi)統(tong)(tong)(tong)(tong)。其中(zhong)基(ji)(ji)因(yin)(yin)擴(kuo)增熱(re)(re)循(xun)環(huan)系(xi)(xi)統(tong)(tong)(tong)(tong)工(gong)(gong)作(zuo)原理與傳統(tong)(tong)(tong)(tong)基(ji)(ji)因(yin)(yin)擴(kuo)增儀(yi)工(gong)(gong)作(zuo)原理基(ji)(ji)本相同,采用半導體(ti)加熱(re)(re)制(zhi)冷工(gong)(gong)作(zuo)方式完成(cheng)熱(re)(re)循(xun)環(huan)過程(cheng)。熒(ying)(ying)光檢(jian)(jian)測系(xi)(xi)統(tong)(tong)(tong)(tong)主(zhu)要(yao)(yao)有(you)由(you)熒(ying)(ying)光激發部件(jian)、光信(xin)號傳輸部件(jian)、熒(ying)(ying)光檢(jian)(jian)測部件(jian)、控制(zhi)系(xi)(xi)統(tong)(tong)(tong)(tong)組(zu)成(cheng)。
三、設備參數: |
外(wai)形尺寸:235mm*385mm*175mm(寬*深*高)
重量:5.6kg
電氣參(can)數(shu):~220V/50Hz,255W
數(shu)據接口(kou):USB 2.0 *2(右側兩個)
環境參(can)數:運行條件:溫度:10-30°C,濕(shi)度:20%~80%
運(yun)輸及貯(zhu)存條件(jian):溫度:-20~55°C,濕度:20%~80%
海拔高度:<2500米(mi)
噪聲等級:A計(ji)權,<60dB
樣本參數:樣本容(rong)量:16*0.2mL試(shi)管類型(xing):單管,八聯排,16孔試(shi)管
樣本容(rong)積(ji):15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫(wen)度(du)范圍:4°C-99°C
最大升溫速(su)率:≥3.5°C/s
平均升溫速率:≥2.5°C/s
最大降溫速率:≥3.5°C/s
平均(jun)降溫速率:≥2.5°C/s
控(kong)溫精度:≤±0.01°C
溫度(du)準確(que)度(du):≤±0.1°C
溫度均勻性:≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光(guang)器件:高亮度LED
采光(guang)器(qi)件:高(gao)靈敏度,高(gao)信噪比光(guang)電二極管
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第(di)二(er)通道(dao):530/570 HEX,JOE,VIC
檢測靈(ling)敏度:1個(ge)拷貝
線性檢(jian)測范圍:100~1010個(ge)拷貝
線性相關(guan)系數:≥0.999
通道交叉串(chuan)擾(rao):無串(chuan)擾(rao)
檢測重復性:≤1.0%