一、概述: |
一般來(lai)說,雄性(xing)(xing)鴿(ge)(ge)子(zi)(zi)(zi)具有(you)(you)ZZ染(ran)色(se)體,而(er)雌性(xing)(xing)鴿(ge)(ge)子(zi)(zi)(zi)則具有(you)(you)ZW染(ran)色(se)體。鴿(ge)(ge)子(zi)(zi)(zi)的(de)性(xing)(xing)別(bie)(bie)(bie)通常(chang)無(wu)法(fa)(fa)憑肉眼外觀直接確定(ding)(ding),因為(wei)它(ta)們沒(mei)有(you)(you)明顯(xian)的(de)性(xing)(xing)別(bie)(bie)(bie)特(te)征。然而(er),通過(guo)基因檢(jian)測提取鴿(ge)(ge)子(zi)(zi)(zi)的(de)DNA樣本,可以進(jin)行基因檢(jian)測來(lai)確定(ding)(ding)其性(xing)(xing)別(bie)(bie)(bie)。這(zhe)種方法(fa)(fa)利用(yong)了鴿(ge)(ge)子(zi)(zi)(zi)的(de)性(xing)(xing)染(ran)色(se)體差異(yi)。通過(guo)檢(jian)測特(te)定(ding)(ding)基因片段的(de)存在(zai)與否,可以確定(ding)(ding)鴿(ge)(ge)子(zi)(zi)(zi)的(de)性(xing)(xing)別(bie)(bie)(bie)。
二、儀器基本結構: |
鴿(ge)子性(xing)別檢測(ce)儀是(shi)實(shi)時檢測(ce)反應(ying)的(de)儀器,主要由基因擴增熱(re)(re)循環(huan)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)、熒光(guang)(guang)實(shi)時檢測(ce)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)、微電路控制(zhi)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)、計(ji)算機及(ji)應(ying)用(yong)軟件組成(cheng)(cheng)。其中(zhong)兩個核(he)心功能模塊:熱(re)(re)循環(huan)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)和熒光(guang)(guang)實(shi)時檢測(ce)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)。其中(zhong)基因擴增熱(re)(re)循環(huan)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)工(gong)作原理與傳(chuan)統(tong)(tong)(tong)(tong)基因擴增儀工(gong)作原理基本相同,采用(yong)半導(dao)體加(jia)熱(re)(re)制(zhi)冷工(gong)作方式完成(cheng)(cheng)熱(re)(re)循環(huan)過(guo)程。熒光(guang)(guang)檢測(ce)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)主要有由熒光(guang)(guang)激發(fa)部(bu)件、光(guang)(guang)信號傳(chuan)輸部(bu)件、熒光(guang)(guang)檢測(ce)部(bu)件、控制(zhi)系(xi)(xi)(xi)統(tong)(tong)(tong)(tong)組成(cheng)(cheng)。
三、設備參數: |
外形(xing)尺寸:235mm*385mm*175mm(寬*深*高)
重量:5.6kg
電氣參數:~220V/50Hz,255W
數(shu)據接口:USB 2.0 *2(右側兩個)
環境參(can)數(shu):運行條件:溫度:10-30°C,濕(shi)度:20%~80%
運輸(shu)及貯存條件:溫度(du):-20~55°C,濕度(du):20%~80%
海拔(ba)高(gao)度:<2500米
噪聲等級:A計權(quan),<60dB
樣本參數:樣本容量:16*0.2mL試管類型:單(dan)管,八聯排,16孔試管
樣(yang)本容積:15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫度范圍:4°C-99°C
最(zui)大升溫速率:≥3.5°C/s
平均升溫速率:≥2.5°C/s
最大降溫速率(lv):≥3.5°C/s
平均降溫速(su)率(lv):≥2.5°C/s
控溫精度(du):≤±0.01°C
溫度準確(que)度:≤±0.1°C
溫度均勻性(xing):≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光(guang)器件:高亮(liang)度LED
采光器件(jian):高靈敏度,高信噪比光電二(er)極管
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通(tong)道:530/570 HEX,JOE,VIC
檢測靈敏度(du):1個(ge)拷貝
線性檢測范圍(wei):100~1010個拷(kao)貝
線(xian)性相關系數(shu):≥0.999
通(tong)道交叉串擾:無串擾
檢(jian)測重(zhong)復性:≤1.0%