一、概述: |
一般來說,雄性(xing)(xing)鴿(ge)子(zi)(zi)具(ju)有ZZ染(ran)色體,而(er)(er)雌性(xing)(xing)鴿(ge)子(zi)(zi)則具(ju)有ZW染(ran)色體。鴿(ge)子(zi)(zi)的(de)(de)(de)性(xing)(xing)別(bie)(bie)(bie)通(tong)常無(wu)法(fa)憑肉眼(yan)外觀直(zhi)接確定(ding),因為它們(men)沒(mei)有明顯的(de)(de)(de)性(xing)(xing)別(bie)(bie)(bie)特征。然而(er)(er),通(tong)過(guo)基(ji)(ji)因檢(jian)測(ce)(ce)提取鴿(ge)子(zi)(zi)的(de)(de)(de)DNA樣(yang)本,可以進行基(ji)(ji)因檢(jian)測(ce)(ce)來確定(ding)其性(xing)(xing)別(bie)(bie)(bie)。這種方法(fa)利(li)用了鴿(ge)子(zi)(zi)的(de)(de)(de)性(xing)(xing)染(ran)色體差(cha)異(yi)。通(tong)過(guo)檢(jian)測(ce)(ce)特定(ding)基(ji)(ji)因片段的(de)(de)(de)存(cun)在與否,可以確定(ding)鴿(ge)子(zi)(zi)的(de)(de)(de)性(xing)(xing)別(bie)(bie)(bie)。
二、儀器基本結構: |
鴿(ge)子性(xing)別檢(jian)測儀是實(shi)時檢(jian)測反應的儀器,主(zhu)要(yao)由(you)基因擴增熱(re)循(xun)(xun)環(huan)系(xi)統(tong)(tong)(tong)、熒(ying)光(guang)(guang)(guang)實(shi)時檢(jian)測系(xi)統(tong)(tong)(tong)、微電路控(kong)(kong)制系(xi)統(tong)(tong)(tong)、計(ji)算機及(ji)應用軟件組(zu)成(cheng)。其中兩個(ge)核心(xin)功(gong)能模塊:熱(re)循(xun)(xun)環(huan)系(xi)統(tong)(tong)(tong)和熒(ying)光(guang)(guang)(guang)實(shi)時檢(jian)測系(xi)統(tong)(tong)(tong)。其中基因擴增熱(re)循(xun)(xun)環(huan)系(xi)統(tong)(tong)(tong)工(gong)(gong)作原理(li)與傳統(tong)(tong)(tong)基因擴增儀工(gong)(gong)作原理(li)基本相同(tong),采用半導體加熱(re)制冷(leng)工(gong)(gong)作方式完成(cheng)熱(re)循(xun)(xun)環(huan)過程。熒(ying)光(guang)(guang)(guang)檢(jian)測系(xi)統(tong)(tong)(tong)主(zhu)要(yao)有由(you)熒(ying)光(guang)(guang)(guang)激發部件、光(guang)(guang)(guang)信號傳輸(shu)部件、熒(ying)光(guang)(guang)(guang)檢(jian)測部件、控(kong)(kong)制系(xi)統(tong)(tong)(tong)組(zu)成(cheng)。
三、設備參數: |
外(wai)形尺寸:235mm*385mm*175mm(寬*深*高)
重量:5.6kg
電氣(qi)參(can)數(shu):~220V/50Hz,255W
數據(ju)接口:USB 2.0 *2(右側兩個)
環境(jing)參數:運行條件:溫度(du):10-30°C,濕度(du):20%~80%
運輸及貯存條件:溫度:-20~55°C,濕度:20%~80%
海(hai)拔高(gao)度:<2500米(mi)
噪聲等(deng)級:A計權,<60dB
樣本參(can)數(shu):樣本容量:16*0.2mL試管類(lei)型:單管,八聯排,16孔試管
樣本容積:15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫度(du)范(fan)圍:4°C-99°C
最(zui)大升溫速(su)率:≥3.5°C/s
平均升溫速(su)率:≥2.5°C/s
最大(da)降溫速率:≥3.5°C/s
平均降溫(wen)速率:≥2.5°C/s
控溫精度(du):≤±0.01°C
溫度準確度:≤±0.1°C
溫度均勻(yun)性(xing):≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光器件:高亮度LED
采光器件:高靈敏度,高信噪比光電二極管(guan)
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通道:530/570 HEX,JOE,VIC
檢測靈敏度:1個拷貝(bei)
線性(xing)檢測范圍(wei):100~1010個拷貝
線(xian)性相關(guan)系數:≥0.999
通道交叉串(chuan)擾:無(wu)串(chuan)擾
檢(jian)測重(zhong)復性:≤1.0%