簡要描述: 鴿子(zi)公(gong)母DNA鑒定(ding)儀(yi)IN-P162是實(shi)時檢(jian)測反應的(de)(de)儀(yi)器,該儀(yi)器可以(yi)通(tong)過基因檢(jian)測提取鴿子(zi)的(de)(de)DNA樣本,然后進行(xing)基因檢(jian)測來確定(ding)其性(xing)(xing)(xing)別。這種(zhong)方法利用了鴿子(zi)的(de)(de)性(xing)(xing)(xing)染色(se)體差(cha)異。通(tong)過檢(jian)測特定(ding)基因片(pian)段的(de)(de)存(cun)在與否,可以(yi)確定(ding)鴿子(zi)的(de)(de)性(xing)(xing)(xing)別。
一、概述: |
一般來說,雄(xiong)性(xing)鴿(ge)(ge)子具(ju)有(you)ZZ染色(se)體(ti),而雌性(xing)鴿(ge)(ge)子則具(ju)有(you)ZW染色(se)體(ti)。鴿(ge)(ge)子的(de)(de)性(xing)別通(tong)(tong)常無法憑(ping)肉眼外(wai)觀直接確定(ding)(ding),因(yin)為它們(men)沒有(you)明顯的(de)(de)性(xing)別特征。然而,通(tong)(tong)過(guo)基(ji)因(yin)檢測(ce)提取鴿(ge)(ge)子的(de)(de)DNA樣(yang)本,可(ke)以進(jin)行基(ji)因(yin)檢測(ce)來確定(ding)(ding)其性(xing)別。這(zhe)種方法利用(yong)了鴿(ge)(ge)子的(de)(de)性(xing)染色(se)體(ti)差異。通(tong)(tong)過(guo)檢測(ce)特定(ding)(ding)基(ji)因(yin)片段(duan)的(de)(de)存(cun)在與否,可(ke)以確定(ding)(ding)鴿(ge)(ge)子的(de)(de)性(xing)別。
二、儀器基本結構: |
鴿子性別檢(jian)(jian)測(ce)儀是(shi)實時檢(jian)(jian)測(ce)反(fan)應的儀器(qi),主(zhu)要由基(ji)因擴增熱(re)(re)循(xun)(xun)環(huan)系(xi)統(tong)(tong)(tong)(tong)(tong)、熒(ying)光(guang)實時檢(jian)(jian)測(ce)系(xi)統(tong)(tong)(tong)(tong)(tong)、微電(dian)路控制(zhi)系(xi)統(tong)(tong)(tong)(tong)(tong)、計算機及應用軟件組成(cheng)(cheng)。其中(zhong)兩個核心功能模塊(kuai):熱(re)(re)循(xun)(xun)環(huan)系(xi)統(tong)(tong)(tong)(tong)(tong)和熒(ying)光(guang)實時檢(jian)(jian)測(ce)系(xi)統(tong)(tong)(tong)(tong)(tong)。其中(zhong)基(ji)因擴增熱(re)(re)循(xun)(xun)環(huan)系(xi)統(tong)(tong)(tong)(tong)(tong)工作原理與傳統(tong)(tong)(tong)(tong)(tong)基(ji)因擴增儀工作原理基(ji)本相同,采用半導體加熱(re)(re)制(zhi)冷(leng)工作方(fang)式完成(cheng)(cheng)熱(re)(re)循(xun)(xun)環(huan)過(guo)程。熒(ying)光(guang)檢(jian)(jian)測(ce)系(xi)統(tong)(tong)(tong)(tong)(tong)主(zhu)要有由熒(ying)光(guang)激發部件、光(guang)信號傳輸(shu)部件、熒(ying)光(guang)檢(jian)(jian)測(ce)部件、控制(zhi)系(xi)統(tong)(tong)(tong)(tong)(tong)組成(cheng)(cheng)。
三、設備參數: |
外形(xing)尺(chi)寸(cun):235mm*385mm*175mm(寬*深*高)
重量:5.6kg
電氣參數:~220V/50Hz,255W
數據(ju)接口:USB 2.0 *2(右(you)側(ce)兩個(ge))
環境(jing)參(can)數:運行條件:溫度(du):10-30°C,濕度(du):20%~80%
運輸及貯存(cun)條件:溫度:-20~55°C,濕度:20%~80%
海拔高度:<2500米
噪聲等(deng)級:A計權(quan),<60dB
樣(yang)本(ben)參數:樣(yang)本(ben)容量(liang):16*0.2mL試管(guan)(guan)類型:單管(guan)(guan),八聯排,16孔試管(guan)(guan)
樣本容(rong)積(ji):15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫度范圍:4°C-99°C
最大升(sheng)溫速率:≥3.5°C/s
平均升(sheng)溫速(su)率:≥2.5°C/s
最大降溫速率:≥3.5°C/s
平均降溫速率:≥2.5°C/s
控溫精(jing)度:≤±0.01°C
溫度準(zhun)確度:≤±0.1°C
溫(wen)度均勻性(xing):≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光(guang)器件:高亮度LED
采(cai)光器(qi)件:高(gao)靈(ling)敏(min)度(du),高(gao)信噪比光電(dian)二(er)極管
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通道:530/570 HEX,JOE,VIC
檢測靈敏(min)度:1個拷貝
線性檢測范(fan)圍:100~1010個拷貝
線性相關系(xi)數:≥0.999
通道交(jiao)叉串(chuan)(chuan)擾:無串(chuan)(chuan)擾
檢測重復(fu)性:≤1.0%