簡要描述(shu): 鴿(ge)子公母DNA鑒(jian)定(ding)儀(yi)(yi)IN-P162是實(shi)時檢(jian)測(ce)(ce)反(fan)應的(de)(de)(de)儀(yi)(yi)器(qi),該儀(yi)(yi)器(qi)可以通(tong)過基(ji)因檢(jian)測(ce)(ce)提取(qu)鴿(ge)子的(de)(de)(de)DNA樣本,然后進行基(ji)因檢(jian)測(ce)(ce)來(lai)確定(ding)其(qi)性(xing)別。這(zhe)種方(fang)法利(li)用了(le)鴿(ge)子的(de)(de)(de)性(xing)染(ran)色體差異。通(tong)過檢(jian)測(ce)(ce)特定(ding)基(ji)因片段的(de)(de)(de)存在(zai)與否,可以確定(ding)鴿(ge)子的(de)(de)(de)性(xing)別。
一、概述: |
一(yi)般來說,雄(xiong)性(xing)(xing)(xing)鴿子(zi)具(ju)有ZZ染(ran)色體,而雌性(xing)(xing)(xing)鴿子(zi)則具(ju)有ZW染(ran)色體。鴿子(zi)的性(xing)(xing)(xing)別通(tong)常無法憑(ping)肉眼外(wai)觀直接確定(ding),因(yin)為(wei)它們沒有明顯的性(xing)(xing)(xing)別特征。然而,通(tong)過基(ji)(ji)因(yin)檢測(ce)提取鴿子(zi)的DNA樣本(ben),可以(yi)進行基(ji)(ji)因(yin)檢測(ce)來確定(ding)其性(xing)(xing)(xing)別。這種(zhong)方法利用了鴿子(zi)的性(xing)(xing)(xing)染(ran)色體差(cha)異。通(tong)過檢測(ce)特定(ding)基(ji)(ji)因(yin)片段(duan)的存在(zai)與否(fou),可以(yi)確定(ding)鴿子(zi)的性(xing)(xing)(xing)別。
二、儀器基本結構: |
鴿子性別檢(jian)(jian)測(ce)(ce)(ce)(ce)儀(yi)(yi)是實(shi)時檢(jian)(jian)測(ce)(ce)(ce)(ce)反應的(de)儀(yi)(yi)器,主要由基(ji)因(yin)擴增(zeng)熱循(xun)(xun)環(huan)(huan)(huan)系(xi)統(tong)(tong)(tong)(tong)(tong)、熒(ying)(ying)光(guang)實(shi)時檢(jian)(jian)測(ce)(ce)(ce)(ce)系(xi)統(tong)(tong)(tong)(tong)(tong)、微(wei)電(dian)路控制(zhi)系(xi)統(tong)(tong)(tong)(tong)(tong)、計算(suan)機及應用(yong)軟件(jian)組(zu)成。其中(zhong)兩個核心功能模(mo)塊(kuai):熱循(xun)(xun)環(huan)(huan)(huan)系(xi)統(tong)(tong)(tong)(tong)(tong)和熒(ying)(ying)光(guang)實(shi)時檢(jian)(jian)測(ce)(ce)(ce)(ce)系(xi)統(tong)(tong)(tong)(tong)(tong)。其中(zhong)基(ji)因(yin)擴增(zeng)熱循(xun)(xun)環(huan)(huan)(huan)系(xi)統(tong)(tong)(tong)(tong)(tong)工作原理與傳統(tong)(tong)(tong)(tong)(tong)基(ji)因(yin)擴增(zeng)儀(yi)(yi)工作原理基(ji)本相(xiang)同,采(cai)用(yong)半導體(ti)加熱制(zhi)冷工作方式(shi)完成熱循(xun)(xun)環(huan)(huan)(huan)過程(cheng)。熒(ying)(ying)光(guang)檢(jian)(jian)測(ce)(ce)(ce)(ce)系(xi)統(tong)(tong)(tong)(tong)(tong)主要有由熒(ying)(ying)光(guang)激發部(bu)件(jian)、光(guang)信號傳輸部(bu)件(jian)、熒(ying)(ying)光(guang)檢(jian)(jian)測(ce)(ce)(ce)(ce)部(bu)件(jian)、控制(zhi)系(xi)統(tong)(tong)(tong)(tong)(tong)組(zu)成。
三、設備參數: |
外形(xing)尺寸:235mm*385mm*175mm(寬*深*高)
重量:5.6kg
電氣(qi)參數:~220V/50Hz,255W
數據接口:USB 2.0 *2(右側兩(liang)個)
環(huan)境參數(shu):運行條件(jian):溫度(du):10-30°C,濕度(du):20%~80%
運輸及貯存條件:溫(wen)度:-20~55°C,濕度:20%~80%
海拔高度(du):<2500米
噪聲等(deng)級:A計權,<60dB
樣(yang)本(ben)參數:樣(yang)本(ben)容量(liang):16*0.2mL試(shi)管(guan)(guan)類型:單管(guan)(guan),八聯排,16孔試(shi)管(guan)(guan)
樣本容積(ji):15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫度范圍:4°C-99°C
最(zui)大升溫(wen)速率:≥3.5°C/s
平均升溫速率:≥2.5°C/s
最大降溫速率:≥3.5°C/s
平均降溫速率:≥2.5°C/s
控(kong)溫精度:≤±0.01°C
溫度準確度:≤±0.1°C
溫度均(jun)勻性(xing):≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光器件:高亮度LED
采光(guang)器件:高靈敏(min)度,高信噪比光(guang)電二極管
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通道:530/570 HEX,JOE,VIC
檢(jian)測靈敏(min)度(du):1個(ge)拷貝
線性檢(jian)測范圍:100~1010個拷貝(bei)
線性相關系數:≥0.999
通(tong)道交叉串(chuan)擾:無(wu)串(chuan)擾
檢測重復(fu)性:≤1.0%