簡要(yao)描述(shu): 鴿子公母(mu)DNA鑒定(ding)儀(yi)(yi)IN-P162是實時檢測反應的儀(yi)(yi)器,該儀(yi)(yi)器可以通過基(ji)(ji)因(yin)(yin)檢測提取鴿子的DNA樣本,然(ran)后進行基(ji)(ji)因(yin)(yin)檢測來確定(ding)其性別。這種方法利(li)用了鴿子的性染色體差異。通過檢測特定(ding)基(ji)(ji)因(yin)(yin)片(pian)段(duan)的存在(zai)與否,可以確定(ding)鴿子的性別。
一、概述: |
一(yi)般(ban)來說,雄性(xing)鴿(ge)(ge)子具有ZZ染(ran)色(se)(se)體(ti),而(er)雌(ci)性(xing)鴿(ge)(ge)子則具有ZW染(ran)色(se)(se)體(ti)。鴿(ge)(ge)子的性(xing)別(bie)通(tong)常無法(fa)憑肉(rou)眼外觀直接(jie)確定(ding)(ding),因為它們沒有明顯的性(xing)別(bie)特征。然而(er),通(tong)過(guo)(guo)基(ji)因檢測提取鴿(ge)(ge)子的DNA樣(yang)本,可以進行基(ji)因檢測來確定(ding)(ding)其性(xing)別(bie)。這(zhe)種方法(fa)利用(yong)了鴿(ge)(ge)子的性(xing)染(ran)色(se)(se)體(ti)差異。通(tong)過(guo)(guo)檢測特定(ding)(ding)基(ji)因片(pian)段的存在與(yu)否,可以確定(ding)(ding)鴿(ge)(ge)子的性(xing)別(bie)。
二、儀器基本結構: |
鴿(ge)子(zi)性(xing)別檢(jian)(jian)(jian)測儀(yi)是實時檢(jian)(jian)(jian)測反(fan)應(ying)的儀(yi)器(qi),主要(yao)由(you)基(ji)(ji)因(yin)擴增熱(re)(re)(re)循(xun)環(huan)系統(tong)(tong)(tong)(tong)(tong)(tong)、熒(ying)光(guang)實時檢(jian)(jian)(jian)測系統(tong)(tong)(tong)(tong)(tong)(tong)、微電(dian)路控制系統(tong)(tong)(tong)(tong)(tong)(tong)、計算機及應(ying)用軟件組成。其中(zhong)(zhong)兩個核(he)心功能模(mo)塊(kuai):熱(re)(re)(re)循(xun)環(huan)系統(tong)(tong)(tong)(tong)(tong)(tong)和熒(ying)光(guang)實時檢(jian)(jian)(jian)測系統(tong)(tong)(tong)(tong)(tong)(tong)。其中(zhong)(zhong)基(ji)(ji)因(yin)擴增熱(re)(re)(re)循(xun)環(huan)系統(tong)(tong)(tong)(tong)(tong)(tong)工(gong)作(zuo)(zuo)原(yuan)理與傳(chuan)統(tong)(tong)(tong)(tong)(tong)(tong)基(ji)(ji)因(yin)擴增儀(yi)工(gong)作(zuo)(zuo)原(yuan)理基(ji)(ji)本相同,采用半(ban)導體加熱(re)(re)(re)制冷工(gong)作(zuo)(zuo)方(fang)式完成熱(re)(re)(re)循(xun)環(huan)過程(cheng)。熒(ying)光(guang)檢(jian)(jian)(jian)測系統(tong)(tong)(tong)(tong)(tong)(tong)主要(yao)有(you)由(you)熒(ying)光(guang)激發部件、光(guang)信號傳(chuan)輸部件、熒(ying)光(guang)檢(jian)(jian)(jian)測部件、控制系統(tong)(tong)(tong)(tong)(tong)(tong)組成。
三、設備參數: |
外形尺寸:235mm*385mm*175mm(寬*深(shen)*高)
重量:5.6kg
電氣(qi)參數:~220V/50Hz,255W
數(shu)據接(jie)口:USB 2.0 *2(右(you)側(ce)兩個(ge))
環境參數:運(yun)行(xing)條(tiao)件:溫度(du):10-30°C,濕度(du):20%~80%
運輸及貯存條件:溫度:-20~55°C,濕度:20%~80%
海拔高度(du):<2500米
噪聲等級:A計(ji)權,<60dB
樣本參(can)數:樣本容(rong)量:16*0.2mL試管(guan)類型:單管(guan),八聯排,16孔試管(guan)
樣本(ben)容積:15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫(wen)度范圍:4°C-99°C
最(zui)大升溫速率:≥3.5°C/s
平均升(sheng)溫(wen)速率:≥2.5°C/s
最(zui)大降溫(wen)速率:≥3.5°C/s
平(ping)均降(jiang)溫速率:≥2.5°C/s
控溫精度:≤±0.01°C
溫度(du)準確度(du):≤±0.1°C
溫度均勻(yun)性:≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光器件:高亮(liang)度LED
采光(guang)器件:高靈(ling)敏度,高信噪(zao)比光(guang)電二極管
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通道:530/570 HEX,JOE,VIC
檢測(ce)靈(ling)敏(min)度:1個(ge)拷貝
線性檢測范(fan)圍:100~1010個(ge)拷貝
線性相關系數(shu):≥0.999
通道交叉串(chuan)擾:無串(chuan)擾
檢(jian)測重(zhong)復性:≤1.0%