簡要描述: 鴿(ge)(ge)子(zi)公(gong)母(mu)DNA鑒(jian)定儀(yi)IN-P162是實時檢(jian)(jian)測反應的(de)儀(yi)器,該儀(yi)器可以通(tong)過基(ji)因檢(jian)(jian)測提取(qu)鴿(ge)(ge)子(zi)的(de)DNA樣(yang)本,然后進(jin)行(xing)基(ji)因檢(jian)(jian)測來確(que)定其性(xing)別(bie)。這種方法利(li)用(yong)了鴿(ge)(ge)子(zi)的(de)性(xing)染色(se)體差異。通(tong)過檢(jian)(jian)測特定基(ji)因片段的(de)存在與否(fou),可以確(que)定鴿(ge)(ge)子(zi)的(de)性(xing)別(bie)。
一、概述: |
一(yi)般來說,雄性(xing)鴿(ge)子(zi)具有(you)ZZ染色(se)體,而雌性(xing)鴿(ge)子(zi)則具有(you)ZW染色(se)體。鴿(ge)子(zi)的性(xing)別(bie)通(tong)(tong)常無法(fa)(fa)憑肉眼外觀直(zhi)接確定(ding)(ding)(ding),因(yin)為它們沒(mei)有(you)明顯的性(xing)別(bie)特征。然而,通(tong)(tong)過基(ji)因(yin)檢(jian)測提取鴿(ge)子(zi)的DNA樣本,可以進行基(ji)因(yin)檢(jian)測來確定(ding)(ding)(ding)其性(xing)別(bie)。這種方法(fa)(fa)利(li)用了鴿(ge)子(zi)的性(xing)染色(se)體差異。通(tong)(tong)過檢(jian)測特定(ding)(ding)(ding)基(ji)因(yin)片段的存在與否,可以確定(ding)(ding)(ding)鴿(ge)子(zi)的性(xing)別(bie)。
二、儀器基本結構: |
鴿子性(xing)別檢(jian)測(ce)儀是實(shi)時檢(jian)測(ce)反應的儀器,主要(yao)由(you)基(ji)(ji)(ji)因擴增熱(re)(re)(re)循(xun)環系(xi)(xi)統(tong)(tong)(tong)(tong)、熒(ying)光(guang)(guang)(guang)實(shi)時檢(jian)測(ce)系(xi)(xi)統(tong)(tong)(tong)(tong)、微電(dian)路控制(zhi)(zhi)系(xi)(xi)統(tong)(tong)(tong)(tong)、計算機及應用軟(ruan)件組(zu)成。其中兩(liang)個核心功能(neng)模塊:熱(re)(re)(re)循(xun)環系(xi)(xi)統(tong)(tong)(tong)(tong)和(he)熒(ying)光(guang)(guang)(guang)實(shi)時檢(jian)測(ce)系(xi)(xi)統(tong)(tong)(tong)(tong)。其中基(ji)(ji)(ji)因擴增熱(re)(re)(re)循(xun)環系(xi)(xi)統(tong)(tong)(tong)(tong)工(gong)作(zuo)原(yuan)理與傳統(tong)(tong)(tong)(tong)基(ji)(ji)(ji)因擴增儀工(gong)作(zuo)原(yuan)理基(ji)(ji)(ji)本相同(tong),采用半導體加熱(re)(re)(re)制(zhi)(zhi)冷(leng)工(gong)作(zuo)方式完(wan)成熱(re)(re)(re)循(xun)環過程(cheng)。熒(ying)光(guang)(guang)(guang)檢(jian)測(ce)系(xi)(xi)統(tong)(tong)(tong)(tong)主要(yao)有由(you)熒(ying)光(guang)(guang)(guang)激發部件、光(guang)(guang)(guang)信號傳輸(shu)部件、熒(ying)光(guang)(guang)(guang)檢(jian)測(ce)部件、控制(zhi)(zhi)系(xi)(xi)統(tong)(tong)(tong)(tong)組(zu)成。
三、設備參數: |
外(wai)形尺(chi)寸:235mm*385mm*175mm(寬*深*高)
重量:5.6kg
電氣參數:~220V/50Hz,255W
數據接(jie)口(kou):USB 2.0 *2(右(you)側兩個(ge))
環境參(can)數(shu):運行條件:溫度:10-30°C,濕度:20%~80%
運(yun)輸及貯存條件:溫度:-20~55°C,濕度:20%~80%
海拔高(gao)度:<2500米
噪聲等級:A計權,<60dB
樣本(ben)參數:樣本(ben)容量:16*0.2mL試管(guan)類(lei)型:單管(guan),八聯排,16孔(kong)試管(guan)
樣本容積:15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫度范圍:4°C-99°C
最大升(sheng)溫(wen)速(su)率:≥3.5°C/s
平(ping)均升(sheng)溫(wen)速率:≥2.5°C/s
最(zui)大降溫速率:≥3.5°C/s
平均降(jiang)溫(wen)速(su)率(lv):≥2.5°C/s
控(kong)溫精度:≤±0.01°C
溫度準確度:≤±0.1°C
溫(wen)度(du)均勻性:≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光(guang)器件:高亮度LED
采(cai)光器(qi)件:高靈敏度,高信(xin)噪(zao)比(bi)光電二極管(guan)
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通道:530/570 HEX,JOE,VIC
檢測靈(ling)敏度:1個拷貝
線性檢(jian)測范圍:100~1010個拷貝
線性相關系數:≥0.999
通道交叉串擾(rao):無串擾(rao)
檢測重復性:≤1.0%