簡要描述: 鴿子(zi)公母DNA鑒定(ding)儀IN-P162是(shi)實時(shi)檢(jian)(jian)測(ce)(ce)反應的(de)儀器,該儀器可以通(tong)過基(ji)因(yin)檢(jian)(jian)測(ce)(ce)提取鴿子(zi)的(de)DNA樣本,然后進行基(ji)因(yin)檢(jian)(jian)測(ce)(ce)來確定(ding)其性別。這種方法利(li)用了鴿子(zi)的(de)性染(ran)色體差異。通(tong)過檢(jian)(jian)測(ce)(ce)特定(ding)基(ji)因(yin)片段的(de)存在與(yu)否,可以確定(ding)鴿子(zi)的(de)性別。
一、概述: |
一般來說,雄性(xing)(xing)(xing)鴿(ge)子(zi)(zi)具有ZZ染(ran)色體(ti),而雌性(xing)(xing)(xing)鴿(ge)子(zi)(zi)則具有ZW染(ran)色體(ti)。鴿(ge)子(zi)(zi)的(de)性(xing)(xing)(xing)別(bie)通(tong)常(chang)無法憑肉(rou)眼外觀直接(jie)確定(ding),因(yin)為它們(men)沒(mei)有明(ming)顯的(de)性(xing)(xing)(xing)別(bie)特征(zheng)。然而,通(tong)過基因(yin)檢測(ce)提取鴿(ge)子(zi)(zi)的(de)DNA樣本,可(ke)以進(jin)行基因(yin)檢測(ce)來確定(ding)其(qi)性(xing)(xing)(xing)別(bie)。這種方法利用了鴿(ge)子(zi)(zi)的(de)性(xing)(xing)(xing)染(ran)色體(ti)差異。通(tong)過檢測(ce)特定(ding)基因(yin)片段的(de)存在(zai)與否,可(ke)以確定(ding)鴿(ge)子(zi)(zi)的(de)性(xing)(xing)(xing)別(bie)。
二、儀器基本結構: |
鴿(ge)子性別檢測儀(yi)是(shi)實(shi)時檢測反應的儀(yi)器,主要(yao)由基因擴(kuo)(kuo)增熱(re)(re)循(xun)環(huan)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)、熒(ying)光(guang)實(shi)時檢測系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)、微電路控制(zhi)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)、計算機及應用軟件(jian)組成(cheng)。其(qi)(qi)中兩個核心(xin)功能(neng)模塊:熱(re)(re)循(xun)環(huan)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)和熒(ying)光(guang)實(shi)時檢測系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)。其(qi)(qi)中基因擴(kuo)(kuo)增熱(re)(re)循(xun)環(huan)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)工(gong)作(zuo)原理與(yu)傳統(tong)(tong)(tong)(tong)(tong)(tong)基因擴(kuo)(kuo)增儀(yi)工(gong)作(zuo)原理基本相同(tong),采用半導體加熱(re)(re)制(zhi)冷工(gong)作(zuo)方式完成(cheng)熱(re)(re)循(xun)環(huan)過(guo)程(cheng)。熒(ying)光(guang)檢測系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)主要(yao)有(you)由熒(ying)光(guang)激(ji)發部件(jian)、光(guang)信(xin)號傳輸(shu)部件(jian)、熒(ying)光(guang)檢測部件(jian)、控制(zhi)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)(tong)組成(cheng)。
三、設備參數: |
外形尺寸:235mm*385mm*175mm(寬*深(shen)*高(gao))
重量:5.6kg
電氣參數(shu):~220V/50Hz,255W
數據接口:USB 2.0 *2(右側兩個(ge))
環境參數:運行條件:溫度:10-30°C,濕度:20%~80%
運輸及貯存條(tiao)件:溫度:-20~55°C,濕度:20%~80%
海拔高度:<2500米(mi)
噪聲等級:A計權,<60dB
樣(yang)(yang)本(ben)參數(shu):樣(yang)(yang)本(ben)容量:16*0.2mL試管類型:單管,八聯排,16孔(kong)試管
樣(yang)本容(rong)積(ji):15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫(wen)度(du)范圍(wei):4°C-99°C
最大升溫速率:≥3.5°C/s
平(ping)均升溫速(su)率:≥2.5°C/s
最(zui)大(da)降溫速率:≥3.5°C/s
平均(jun)降溫速(su)率:≥2.5°C/s
控溫精度:≤±0.01°C
溫度(du)準確度(du):≤±0.1°C
溫度均(jun)勻性:≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光(guang)器件:高亮度(du)LED
采光器件:高靈敏(min)度,高信噪比光電二(er)極管
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通道:530/570 HEX,JOE,VIC
檢測靈(ling)敏度:1個拷貝
線性(xing)檢測(ce)范圍(wei):100~1010個拷貝
線性相(xiang)關系數:≥0.999
通道交叉串(chuan)擾(rao):無串(chuan)擾(rao)
檢測重復(fu)性:≤1.0%