簡要描述: 鴿(ge)子(zi)(zi)公母(mu)DNA鑒(jian)定儀IN-P162是實時(shi)檢(jian)(jian)測反(fan)應的儀器,該儀器可以通(tong)過基(ji)(ji)因(yin)檢(jian)(jian)測提取鴿(ge)子(zi)(zi)的DNA樣本,然后進行基(ji)(ji)因(yin)檢(jian)(jian)測來確定其性(xing)別。這種方法利用了鴿(ge)子(zi)(zi)的性(xing)染色體(ti)差異(yi)。通(tong)過檢(jian)(jian)測特(te)定基(ji)(ji)因(yin)片段的存在與否,可以確定鴿(ge)子(zi)(zi)的性(xing)別。
一、概述: |
一般來說,雄性(xing)鴿(ge)子具(ju)有(you)ZZ染色(se)體(ti),而雌性(xing)鴿(ge)子則具(ju)有(you)ZW染色(se)體(ti)。鴿(ge)子的(de)(de)性(xing)別通(tong)常無(wu)法憑肉眼外(wai)觀直接確定(ding)(ding),因(yin)為它們(men)沒有(you)明顯的(de)(de)性(xing)別特(te)征。然而,通(tong)過基(ji)因(yin)檢(jian)測(ce)(ce)提取(qu)鴿(ge)子的(de)(de)DNA樣本(ben),可(ke)以(yi)進(jin)行基(ji)因(yin)檢(jian)測(ce)(ce)來確定(ding)(ding)其性(xing)別。這種方法利用了鴿(ge)子的(de)(de)性(xing)染色(se)體(ti)差(cha)異。通(tong)過檢(jian)測(ce)(ce)特(te)定(ding)(ding)基(ji)因(yin)片段的(de)(de)存在與否(fou),可(ke)以(yi)確定(ding)(ding)鴿(ge)子的(de)(de)性(xing)別。
二、儀器基本結構: |
鴿子性別檢(jian)(jian)測(ce)(ce)儀(yi)是實時檢(jian)(jian)測(ce)(ce)反應的儀(yi)器,主要(yao)由基(ji)因(yin)擴(kuo)增熱(re)(re)循(xun)環(huan)(huan)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)、熒(ying)光(guang)(guang)(guang)(guang)實時檢(jian)(jian)測(ce)(ce)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)、微電路控制(zhi)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)、計算機及(ji)應用(yong)軟件(jian)(jian)組成(cheng)。其(qi)中兩個核心(xin)功能模(mo)塊:熱(re)(re)循(xun)環(huan)(huan)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)和熒(ying)光(guang)(guang)(guang)(guang)實時檢(jian)(jian)測(ce)(ce)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)。其(qi)中基(ji)因(yin)擴(kuo)增熱(re)(re)循(xun)環(huan)(huan)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)工作(zuo)原(yuan)理與傳統(tong)(tong)(tong)(tong)(tong)基(ji)因(yin)擴(kuo)增儀(yi)工作(zuo)原(yuan)理基(ji)本相(xiang)同,采用(yong)半導體加熱(re)(re)制(zhi)冷工作(zuo)方式(shi)完(wan)成(cheng)熱(re)(re)循(xun)環(huan)(huan)過程。熒(ying)光(guang)(guang)(guang)(guang)檢(jian)(jian)測(ce)(ce)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)主要(yao)有由熒(ying)光(guang)(guang)(guang)(guang)激發部件(jian)(jian)、光(guang)(guang)(guang)(guang)信號傳輸部件(jian)(jian)、熒(ying)光(guang)(guang)(guang)(guang)檢(jian)(jian)測(ce)(ce)部件(jian)(jian)、控制(zhi)系(xi)(xi)(xi)(xi)統(tong)(tong)(tong)(tong)(tong)組成(cheng)。
三、設備參數: |
外(wai)形尺(chi)寸:235mm*385mm*175mm(寬*深*高(gao))
重量:5.6kg
電氣參數:~220V/50Hz,255W
數據接口:USB 2.0 *2(右側兩個)
環境參(can)數:運行條件:溫度:10-30°C,濕度:20%~80%
運輸及貯存條件:溫度:-20~55°C,濕度:20%~80%
海(hai)拔高(gao)度:<2500米(mi)
噪聲(sheng)等(deng)級:A計權,<60dB
樣(yang)本參(can)數(shu):樣(yang)本容(rong)量:16*0.2mL試管類(lei)型:單管,八聯排,16孔試管
樣本容積:15-100uL
四、溫度特性: |
加熱/冷卻方式:半導體加熱/制冷
溫度(du)范(fan)圍:4°C-99°C
最(zui)大升溫(wen)速率:≥3.5°C/s
平均(jun)升溫速率:≥2.5°C/s
最大(da)降溫速(su)率:≥3.5°C/s
平均降溫速率:≥2.5°C/s
控溫精度(du):≤±0.01°C
溫(wen)度準確度:≤±0.1°C
溫(wen)度均勻性(xing):≤±0.3°C
五、光學特性: |
熒光檢測通道數:2通道
發光器件:高(gao)亮度LED
采(cai)光器件:高靈敏(min)度(du),高信(xin)噪比光電二(er)極管
六、適配探針或染料: |
第一通道:470/520 FAM,SYBR Green
第二通道:530/570 HEX,JOE,VIC
檢測靈敏度:1個拷貝
線性檢測范圍:100~1010個(ge)拷貝
線性(xing)相關系數(shu):≥0.999
通道交叉串擾:無串擾
檢測重復性:≤1.0%